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細(xì)胞中文名稱(chēng)正常小鼠睪丸Sertoli細(xì)胞
細(xì)胞英文名稱(chēng)TM4
物種小鼠
細(xì)胞形態(tài)特征上皮樣
細(xì)胞生長(zhǎng)特性正常小鼠睪丸Sertoli細(xì)胞TM4細(xì)胞 貼壁生長(zhǎng)
細(xì)胞特種特性 該細(xì)胞在FSH的作用下cAMP產(chǎn)量增加,但對(duì)LH無(wú)反應(yīng);與原代Sertoli細(xì)胞相比,該細(xì)胞對(duì)FSH的反應(yīng)性降低。據(jù)報(bào)道,該細(xì)胞組成性纖溶酶原激活物的產(chǎn)量很低,但可被FSH刺激產(chǎn)生,受維甲酸刺激可有大幅增高。該細(xì)胞可產(chǎn)生視黃醇結(jié)合蛋白、組織纖溶酶原激活物和轉(zhuǎn)鐵蛋白;表達(dá)FSH受體、雄激素受體和孕激素受體。鼠痘病毒陰性
培養(yǎng)基DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium)
血清5%HS + 2.5%FBS
細(xì)胞傳代方法1:10~1:50傳代;3~4天換液1次
細(xì)胞傳代情況C6
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+5%DMSO+20%FBS
支原體檢測(cè)陰性
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