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您當(dāng)前的位置:首頁 > 產(chǎn)品展示 > 實驗細胞 > 細胞 > SV40轉(zhuǎn)染人成骨細胞;hFOB 1.19
SV40轉(zhuǎn)染人成骨細胞;hFOB 1.19
產(chǎn)品時間:2016-06-30
SV40轉(zhuǎn)染人成骨細胞;hFOB 1.19,公司冰爽低價供應(yīng),購買細胞,終身享受公司細胞技術(shù)培養(yǎng)服務(wù),我們可以免費代養(yǎng),不含有細菌、支原體、真菌、酵母、HIV、HBV、HCV、苯、血清、內(nèi)毒素等細胞污染源。

本產(chǎn)品僅用于科研,不用于臨床

SV40轉(zhuǎn)染人成骨細胞;hFOB 1.19
貼壁生長
在0.6mg/ml新霉素G418存在下,用溫度敏感的表達載體pUCSVisA58轉(zhuǎn)染自然流產(chǎn)的胎兒四肢組織,建立了此細胞系。 在許可溫度33.5oC下細胞分裂很快,SV40轉(zhuǎn)染人成骨細胞;hFOB 1.19而在限制溫度39.5oC下細胞極少分裂或不分裂。 這些細胞具有分化為表達造骨細胞表型的成熟造骨細胞的能力。 這些細胞提供了一個同質(zhì)化的快速增殖模型系統(tǒng),用于研究正常人造骨細胞的分化,造骨細胞生理和激素,生長因子,和對造骨細胞的功能及分化有影響的細胞因子。
DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 胎牛血清,10%;0.3mg/ml G418
消化3-5分鐘。1:2。3天內(nèi)可長滿。
不詳
*培養(yǎng)基+8%DMSO

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