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人抗副流感病毒IgM抗體酶免試劑盒,(anti-PIV IgM)ELISA檢測試劑盒 ,英文名:anti-PIV IgM ELISA Kit ,英文縮寫:anti-PIV IgM ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗副流感病毒IgM抗體ELISA檢測試劑盒,anti-PIV IgM 檢測試劑盒, anti-PIV IgM 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人抗副流感病毒IgM抗體酶免試劑盒,(anti-PIV IgM)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
信號傳導(dǎo)轉(zhuǎn)錄激活因子5A(STAT5A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 5A (STAT5A)48T/96TY09485Homo sapiens (Human)
信號傳導(dǎo)轉(zhuǎn)錄激活因子4(STAT4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Transducer And Activator Of Transcription 4 (STAT4)48T/96TY09486Homo sapiens (Human)
Toll樣受體2(TLR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 2 (TLR2)48T/96TY09487Homo sapiens (Human)
骨鈣素(OC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteocalcin (OC)48T/96TY09488Bos taurus; Bovine (Cattle)
UDP葡萄糖神經(jīng)酰胺葡萄糖基轉(zhuǎn)移酶(UGCG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for UDP Glucose Ceramide Glucosyltransferase (UGCG)48T/96TY09489Homo sapiens (Human)
胰島素(INS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Insulin (INS)48T/96TY09490Mus musculus (Mouse)
胱天蛋白酶7(CASP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 7 (CASP7)48T/96TY09491Homo sapiens (Human)
胱天蛋白酶7(CASP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 7 (CASP7)48T/96TY09492Rattus norvegicus (Rat)
胱天蛋白酶7(CASP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caspase 7 (CASP7)48T/96TY09493Mus musculus (Mouse)
激肽釋放酶4(KLK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 4 (KLK4)48T/96TY09494Homo sapiens (Human)
激肽釋放酶4(KLK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 4 (KLK4)48T/96TY09495Rattus norvegicus (Rat)
激肽釋放酶4(KLK4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 4 (KLK4)48T/96TY09496Mus musculus (Mouse)
基質(zhì)金屬蛋白酶23B(MMP23B)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 23B (MMP23B)48T/96TY09497Mus musculus (Mouse)
髓細(xì)胞觸發(fā)受體1(TREM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Triggering Receptor Expressed On Myeloid Cells 1 (TREM1)48T/96TY09498Rattus norvegicus (Rat)
窖蛋白(CAV1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Caveolin 1 (CAV1)48T/96TY09499Homo sapiens (Human)
Ⅰ型膠原α2(COL1α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 2 (COL1a2)48T/96TY09500Homo sapiens (Human)
Ⅰ型膠原α2(COL1α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 2 (COL1a2)48T/96TY09501Rattus norvegicus (Rat)
Ⅰ型膠原α2(COL1α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Collagen Type I Alpha 2 (COL1a2)48T/96TY09502Mus musculus (Mouse)
非神經(jīng)元性烯醇化酶(NNE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Enolase, Non Neuronal (NNE)48T/96TY09503Homo sapiens (Human)
肌肽(CAR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Carnosine (CAR)48T/96TY09504General
防御素β2(DEFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Defensin Beta 2 (DEFb2)48T/96TY09505Homo sapiens (Human)
早期生長應(yīng)答因子2(EGR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Early Growth Response Protein 2 (EGR2)48T/96TY09506Homo sapiens (Human)
脂氧素A4(LXA4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipoxin A4 (LXA4)48T/96TY09507General
胰脂肪酶(PL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lipase, Pancreatic (PL)48T/96TY09508Homo sapiens (Human)
胰淀粉酶α2(AMY2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Alpha 2, Pancreatic (AMY2)48T/96TY09509Homo sapiens (Human)
精氨酰氨基肽酶(RNPEP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Arginyl Aminopeptidase (RNPEP)48T/96TY09510Homo sapiens (Human)
松弛素/胰島素樣肽受體1(RXFP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Relaxin/Insulin Like Family Peptide Receptor 1 (RXFP1)48T/96TY09511Homo sapiens (Human)
抗神經(jīng)節(jié)苷脂M1抗體(Anti-GM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti-Ganglioside M1 Antibody (Anti-GM1)48T/96TY09512Homo sapiens (Human)
絲裂原激活蛋白激酶激活蛋白激酶2(MAPKAPK2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mitogen Activated Protein Kinase Activated Protein Kinase 2 (MAPKAPK2)48T/96TY09513Homo sapiens (Human)
天青殺素1(AZU1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Azurocidin 1 (AZU1)48T/96TY09514Homo sapiens (Human)
γ-促脂解激素(γLPH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Gamma-Lipotropic Hormone (gLPH)48T/96TY09515Homo sapiens (Human)
軟骨糖蛋白39(GP39)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glycoprotein 39, Cartilage (GP39)48T/96TY09516Homo sapiens (Human)
溶酶體關(guān)聯(lián)膜蛋白2(LAMP2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lysosomal Associated Membrane Protein 2 (LAMP2)48T/96TY09517Homo sapiens (Human)
間隙連接蛋白37(CX37)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connexin 37 (CX37)48T/96TY09518Homo sapiens (Human)
間隙連接蛋白40(CX40)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connexin 40 (CX40)48T/96TY09519Homo sapiens (Human)
間隙連接蛋白31(CX31)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Connexin 31 (CX31)48T/96TY09520Homo sapiens (Human)
甘露糖結(jié)合蛋白(MBL)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mannose Binding Lectin (MBL)48T/96TY09521Homo sapiens (Human)
神經(jīng)鈣黏蛋白(CDH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cadherin, Neuronal (CDH2)48T/96TY09522Homo sapiens (Human)
唾液淀粉酶α1(AMY1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amylase Alpha 1, Salivary (AMY1)48T/96TY09523Homo sapiens (Human)
胰彈性蛋白酶(ELA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Elastase 1, Pancreatic (ELA1)48T/96TY09524Homo sapiens (Human)
成骨生長肽(OGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Osteogenic Growth Peptide (OGP)48T/96TY09525Homo sapiens (Human)
白介素1α(IL1α)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 1 Alpha (IL1a)48T/96TY09526Sus scrofa; Porcine (Pig)
硫氧化還原蛋白(Trx)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin (Trx)48T/96TY09527Homo sapiens (Human)
硫氧化還原蛋白(Trx)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin (Trx)48T/96TY09528Rattus norvegicus (Rat)
硫氧化還原蛋白(Trx)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin (Trx)48T/96TY09529Mus musculus (Mouse)
硫氧還蛋白還原酶1(TrxR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin Reductase 1 (TrxR1)48T/96TY09530Homo sapiens (Human)
硫氧還蛋白還原酶1(TrxR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin Reductase 1 (TrxR1)48T/96TY09531Rattus norvegicus (Rat)
硫氧還蛋白還原酶1(TrxR1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thioredoxin Reductase 1 (TrxR1)48T/96TY09532Mus musculus (Mouse)
孤啡肽(OFQ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Orphanin FQ (OFQ)48T/96TY09533Homo sapiens (Human)
孤啡肽(OFQ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Orphanin FQ (OFQ)48T/96TY09534Rattus norvegicus (Rat)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。