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人抗丁型肝炎病毒抗體酶免試劑盒,(anti-HDV)ELISA檢測試劑盒 ,英文名:anti-HDV ELISA Kit ,英文縮寫:anti-HDV ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人抗丁型肝炎病毒抗體ELISA檢測試劑盒,anti-HDV 檢測試劑盒, anti-HDV 檢測試劑盒
ELISA實驗操作較繁雜,操作不當將引起較大的誤差。人抗丁型肝炎病毒抗體酶免試劑盒,(anti-HDV)ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進展或由其他因素影響,某些抗體在機體內(nèi)的含量發(fā)生大幅波動,因此有必要對標本進行預處理,例如對血清標本作適當稀釋,或離心分離血脂等。間接法測定中,標本適當稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標本、加酶結(jié)合物、加底物和加終止液。加標本時必須每份標本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導致錯誤結(jié)果或定量不準。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標本中與反應(yīng)無關(guān)的成分、游離的酶標物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準確判定結(jié)果須使用ELISA測讀儀(酶標儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標儀具有良好的重復性。
孤啡肽(OFQ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Orphanin FQ (OFQ)48T/96TY09535Mus musculus (Mouse)
基質(zhì)金屬蛋白酶1(MMP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 1 (MMP1)48T/96TY09536Oryctolagus cuniculus (Rabbit)
內(nèi)凝集蛋白1(ITLN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inectin 1 (ITLN1)48T/96TY09537Homo sapiens (Human)
內(nèi)凝集蛋白1(ITLN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inectin 1 (ITLN1)48T/96TY09538Mus musculus (Mouse)
內(nèi)凝集蛋白1(ITLN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inectin 1 (ITLN1)48T/96TY09539Rattus norvegicus (Rat)
載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein E (APOE)48T/96TY09540Homo sapiens (Human)
載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein E (APOE)48T/96TY09541Rattus norvegicus (Rat)
載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein E (APOE)48T/96TY09542Mus musculus (Mouse)
過氧化物酶體增殖物激活受體α(PPARα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa)48T/96TY09543Homo sapiens (Human)
過氧化物酶體增殖物激活受體α(PPARα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa)48T/96TY09544Rattus norvegicus (Rat)
過氧化物酶體增殖物激活受體α(PPARα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peroxisome Proliferator Activated Receptor Alpha (PPARa)48T/96TY09545Mus musculus (Mouse)
粘蛋白2(MUC2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 2 (MUC2)48T/96TY09546Homo sapiens (Human)
C反應(yīng)蛋白(CRP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C Reactive Protein (CRP)48T/96TY09547Oryctolagus cuniculus (Rabbit)
白三烯D4(LTD4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Leukotriene D4 (LTD4)48T/96TY09548General
單核細胞趨化蛋白4(MCP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Monocyte Chemotactic Protein 4 (MCP4)48T/96TY09549Homo sapiens (Human)
尿激酶型纖溶酶原激活因子(uPA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Plasminogen Activator, Urokinase (uPA)48T/96TY09550Oryctolagus cuniculus (Rabbit)
內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制因子(Vaspin)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin)48T/96TY09551Homo sapiens (Human)
Preptin蛋白(Preptin)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Preptin48T/96TY09552Homo sapiens (Human)
α1-微球蛋白(α1M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M)48T/96TY09553Homo sapiens (Human)
α1-微球蛋白(α1M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M)48T/96TY09554Rattus norvegicus (Rat)
α1-微球蛋白(α1M)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Alpha-1-Microglobulin/Bikunin Precursor (a1M)48T/96TY09555Mus musculus (Mouse)
鈣調(diào)素(CAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calmodulin (CAM)48T/96TY09556Homo sapiens (Human)
內(nèi)皮型一氧化氮合酶(NOS3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nitric Oxide Synthase 3, Endothelial (NOS3)48T/96TY09557Oryctolagus cuniculus (Rabbit)
細胞色素P450家族成員2E1(CYP2E1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cytochrome P450 2E1 (CYP2E1)48T/96TY09558Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09559Homo sapiens (Human)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09560Rattus norvegicus (Rat)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09561Mus musculus (Mouse)
轉(zhuǎn)化生長因子β2(TGFβ2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta 2 (TGFb2)48T/96TY09562Oryctolagus cuniculus (Rabbit)
鈣網(wǎng)蛋白(CRT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Calreticulin (CRT)48T/96TY09563Homo sapiens (Human)
肽基脯氨酰順反式異構(gòu)酶NIMA相互作用蛋白4(PIN4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidyl Prolyl Cis/Trans Isomerase NIMA Interacting Protein 4 (PIN4)48T/96TY09564Homo sapiens (Human)
鳥氨酸氨基甲酰轉(zhuǎn)移酶(OCT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Ornithine Carbamoyl Transferase (OCT)48T/96TY09565Homo sapiens (Human)
肽基脯氨酰異構(gòu)酶E(PPIE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Peptidylprolyl Isomerase E (PPIE)48T/96TY09566Homo sapiens (Human)
纖調(diào)蛋白(FMOD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibromodulin (FMOD)48T/96TY09567Homo sapiens (Human)
山梨醇脫氫酶(SDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sorbitol Dehydrogenase (SDH)48T/96TY09568Homo sapiens (Human)
基膜聚糖(LUM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Lumican (LUM)48T/96TY09569Homo sapiens (Human)
蛋白二硫化物異構(gòu)酶A3(PDIA3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A3 (PDIA3)48T/96TY09570Homo sapiens (Human)
蛋白二硫化物異構(gòu)酶A2(PDIA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Disulfide Isomerase A2 (PDIA2)48T/96TY09571Homo sapiens (Human)
載脂蛋白B100(APOB100)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein B100 (APOB100)48T/96TY09572Oryctolagus cuniculus (Rabbit)
載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein E (APOE)48T/96TY09573Oryctolagus cuniculus (Rabbit)
載脂蛋白A1(APOA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A1 (APOA1)48T/96TY09574Oryctolagus cuniculus (Rabbit)
白介素2(IL2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 2 (IL2)48T/96TY09575Bos taurus; Bovine (Cattle)
干擾素γ(IFNγ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interferon Gamma (IFNg)48T/96TY09576Bos taurus; Bovine (Cattle)
內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制因子(Vaspin)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin)48T/96TY09577Rattus norvegicus (Rat)
內(nèi)臟脂肪特異性絲氨酸蛋白酶抑制因子(Vaspin)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Visceral Adipose Tissue Derived Serine Protease Inhibitor (Vaspin)48T/96TY09578Mus musculus (Mouse)
凝血酶原片段F1+2(F1+2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prothrombin Fragment 1+2 (F1+2)48T/96TY09579Homo sapiens (Human)
β-位淀粉樣前體蛋白裂解酶1(βACE1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Beta-Site APP Cleaving Enzyme 1 (bACE1)48T/96TY09580Homo sapiens (Human)
次黃嘌呤磷酸核糖基轉(zhuǎn)移酶1(HPRT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)48T/96TY09581Bos taurus; Bovine (Cattle)
葡萄糖6磷酸脫氫酶(G6PD)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose 6 Phosphate Dehydrogenase (G6PD)48T/96TY09582Bos taurus; Bovine (Cattle)
絲氨酸蛋白酶1(PRSS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protease, Serine 1 (PRSS1)48T/96T Y09583Homo sapiens (Human)
磷酸烯醇式丙酮酸羧激酶1(PCK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phosphoenolpyruvate Carboxykinase 1, Soluble (PCK1)48T/96TY09584Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。