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人包蟲抗體IgG ELISA檢測試劑盒,英文名:人包蟲抗體IgG ELISA試劑盒,英文縮寫:人包蟲抗體IgG ELISA試劑盒,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:人包蟲抗體IgG ELISA試劑盒人包蟲抗體IgGELISA試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。人包蟲抗體IgG ELISA檢測試劑盒 在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
細(xì)胞間粘附分子5(ICAM5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Intercellular Adhesion Molecule 5 (ICAM5)48T/96TY09735Oryctolagus cuniculus (Rabbit)
血栓素B2(TXB2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thromboxane B2 (TXB2)48T/96TY09736Oryctolagus cuniculus (Rabbit)
激肽釋放酶10(KLK10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 10 (KLK10)48T/96TY09737Homo sapiens (Human)
激肽釋放酶11(KLK11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Kallikrein 11 (KLK11)48T/96TY09738Homo sapiens (Human)
9kDa信號識別顆粒(SRP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Signal Recognition Particle 9kDa (SRP9)48T/96TY09739Homo sapiens (Human)
粒細(xì)胞特異性抗核抗體(GSANA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Granulocyte Specific Anti-Nuclear Antibody (GSANA)48T/96TY09740Homo sapiens (Human)
甲狀腺激素自身抗體(THAA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thyroid Hormone Autoantibody (THAA)48T/96TY09741Homo sapiens (Human)
周期素依賴性激酶5(CDK5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 5 (CDK5)48T/96TY09742Homo sapiens (Human)
環(huán)加氧酶2(PTGS2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Prostaglandin Endoperoxide Synthase 2 (PTGS2)48T/96TY09743Homo sapiens (Human)
Dickkopf相關(guān)蛋白1(DKK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dickkopf Related Protein 1 (DKK1)48T/96TY09744Homo sapiens (Human)
羥賴氨酸(Hyl)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Hydroxylysine (Hyl)48T/96TY09745General
金屬硫蛋白3(MT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Metallothionein 3 (MT3)48T/96TY09746Bos taurus; Bovine (Cattle)
棕櫚?;さ鞍?(MPP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Membrane Protein, Palmitoylated 5 (MPP5)48T/96TY09747Homo sapiens (Human)
內(nèi)嗎啡肽2(EM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Endomorphin 2 (EM2)48T/96TY09748Homo sapiens (Human)
粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 5 Subtype AC (MUC5AC)48T/96TY09749Homo sapiens (Human)
8-羥基脫氧鳥苷(8-OHdG)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for 8-Hydroxydeoxyguanosine (8-OHdG)48T/96TY09750General
胞漿型磷脂酶A2(cPLA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Phospholipase A2, Cytosolic (cPLA2)48T/96TY09751Homo sapiens (Human)
褪黑素(MT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Melatonin (MT)48T/96TY09752General
Ⅰ型膠原交聯(lián)羧基端肽(CTXⅠ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cross Linked C-opeptide Of Type I Collagen (CTXI)48T/96TY09753Homo sapiens (Human)
葡萄糖激酶(GCK)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucokinase (GCK)48T/96TY09754Homo sapiens (Human)
歸巢關(guān)聯(lián)細(xì)胞黏附分子(HCAM)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Homing Associated Cell Adhesion Molecule (HCAM)48T/96TY09755Rattus norvegicus (Rat)
淀粉樣蛋白β1-42(Aβ1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)48T/96TY09756Homo sapiens (Human)
淀粉樣蛋白β1-42(Aβ1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)48T/96TY09757Rattus norvegicus (Rat)
淀粉樣蛋白β1-42(Aβ1-42)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Amyloid Beta Peptide 1-42 (Ab1-42)48T/96TY09758Mus musculus (Mouse)
胸腺嘧啶核苷磷酸化酶(TP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thymidine Phosphorylase (TP)48T/96TY09759Homo sapiens (Human)
Slit同源物2(Slit2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 2 (Slit2)48T/96TY09760Homo sapiens (Human)
Slit同源物2(Slit2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Slit Homolog 2 (Slit2)48T/96TY09761Mus musculus (Mouse)
粘蛋白5AC(MUC5AC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mucin 5 Subtype AC (MUC5AC)48T/96TY09762Mus musculus (Mouse)
載脂蛋白A2(APOA2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein A2 (APOA2)48T/96TY09763Rhesus monkey (Simian)
載脂蛋白B(APOB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apolipoprotein B (APOB)48T/96TY09764Rattus norvegicus (Rat)
多巴胺受體D2(DRD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D2 (DRD2)48T/96TY09765Rattus norvegicus (Rat)
多巴胺受體D2(DRD2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Receptor D2 (DRD2)48T/96TY09766Homo sapiens (Human)
多巴胺轉(zhuǎn)運蛋白(DAT)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Dopamine Transporter (DAT)48T/96TY09767Rattus norvegicus (Rat)
腫瘤蛋白p53(TP53)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Protein p53 (TP53)48T/96TY09768Oryctolagus cuniculus (Rabbit)
Toll樣受體4(TLR4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Toll Like Receptor 4 (TLR4)48T/96TY09769Rattus norvegicus (Rat)
肌球蛋白重鏈1(MYH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)48T/96TY09770Homo sapiens (Human)
肌球蛋白重鏈1(MYH1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 1, Skeletal Muscle, Adult (MYH1)48T/96TY09771Mus musculus (Mouse)
肌球蛋白重鏈2(MYH2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Myosin Heavy Chain 2, Skeletal Muscle, Adult (MYH2)48T/96TY09772Mus musculus (Mouse)
腫瘤壞死因子受體超家族成員8(TNFRSF8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 8 (TNFRSF8)48T/96TY09773Mus musculus (Mouse)
蛋白激酶Cθ(PKCθ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase C Theta (PKCq)48T/96TY09774Rattus norvegicus (Rat)
叉頭框蛋白M1(FOXM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein M1 (FOXM1)48T/96TY09775Rattus norvegicus (Rat)
表皮生長因子受體(EGFR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Epidermal Growth Factor Receptor (EGFR)48T/96TY09776Homo sapiens (Human)
抗繆勒管激素(AMH)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Anti Mullerian Hormone (AMH)48T/96TY09777Rattus norvegicus (Rat)
補(bǔ)體成分7(C7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement Component 7 (C7)48T/96TY09778Homo sapiens (Human)
補(bǔ)體C3轉(zhuǎn)化酶(C3c)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Complement C3 Convertase (C3c)48T/96TY09779Homo sapiens (Human)
抑制素B(INHB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Inhibin B (INHB)48T/96TY09780Homo sapiens (Human)
C4結(jié)合蛋白β(C4BPβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for C4 Binding Protein Beta (C4BPb)48T/96TY09781Homo sapiens (Human)
基質(zhì)金屬蛋白酶11(MMP11)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Matrix Metalloproteinase 11 (MMP11)48T/96TY09782Homo sapiens (Human)
解整合素金屬蛋白酶10(ADAM10)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for A Disintegrin And Metalloprotease 10 (ADAM10)48T/96T
Y09783Homo sapiens (Human)
AMP激活蛋白激酶α1(PRKAα1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Protein Kinase, AMP Activated Alpha 1 (PRKAa1)48T/96TY09784Homo sapiens (Human)
各種類型ELISA測定時, 一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。