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小鼠胰蛋白酶酶免試劑盒,(trypsin)ELISA檢測試劑盒 ,英文名:trypsin ELISA Kit ,英文縮寫:trypsin ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠胰蛋白酶ELISA檢測試劑盒,trypsin 檢測試劑盒, trypsin 檢測試劑盒
ELISA實驗操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠胰蛋白酶酶免試劑盒,(trypsin)ELISA檢測試劑盒在操作中應(yīng)注意以下5個方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動,因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時差的影響。加樣及混勻時間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實驗成敗的一個關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)??捎孟窗鍣C(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
含IQ基元GTP酶激活蛋白1(IQGAP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for IQ Motif Containing GTPase Activating Protein 1 (IQGAP1)48T/96TY16335Mus musculus (Mouse)
微管蛋白α3A(TUBα3A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tubulin Alpha 4A (TUBa4A)48T/96TY16336Mus musculus (Mouse)
甲狀腺激素受體交互子6(TRIP6)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thyroid Hormone Receptor Interactor 6 (TRIP6)48T/96TY16337Mus musculus (Mouse)
葡萄糖轉(zhuǎn)運(yùn)蛋白1(GLUT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 1 (GLUT1)48T/96TY16338Rattus norvegicus (Rat)
70kDa熱休克蛋白1A(HSPA1A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock 70kDa Protein 1A (HSPA1A)48T/96TY16339Bos taurus; Bovine (Cattle)
煙堿型膽堿受體α7(CHRNα7)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7)48T/96TY16340Homo sapiens (Human)
促甲狀腺素受體(TSHR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Thyroid Stimulating Hormone Receptor (TSHR)48T/96TY16341Rattus norvegicus (Rat)
煙堿型膽堿受體α3(CHRNα3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cholinergic Receptor, Nicotinic, Alpha 3 (CHRNa3)48T/96TY16342Homo sapiens (Human)
腭/肺/鼻上皮癌關(guān)聯(lián)蛋白(PLUNC)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Palate/Lung And Nasal Epithelium Associated Protein (PLUNC)48T/96TY16343Homo sapiens (Human)
GLI家族鋅指蛋白1(GLI1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for GLI Family Zinc Finger Protein 1 (GLI1)48T/96TY16344Homo sapiens (Human)
非轉(zhuǎn)移細(xì)胞1表達(dá)NM23A蛋白(NME1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Non Metastatic Cells 1, Protein NM23A Expressed In (NME1)48T/96TY16345Rattus norvegicus (Rat)
90kDa熱休克蛋白αA1(HSP90αA1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Heat Shock Protein 90kDa Alpha A1 (HSP90aA1)48T/96TY16346Bos taurus; Bovine (Cattle)
成纖維細(xì)胞激活蛋白α(FAPα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Activation Protein Alpha (FAPa)48T/96TY16347Rattus norvegicus (Rat)
黑素瘤缺乏因子2(AIM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Absent In Melanoma 2 (AIM2)48T/96TY16348Homo sapiens (Human)
Apelin 13蛋白(AP13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Apelin 13 (AP13)48T/96TY16349Rattus norvegicus (Rat)
跨膜4域亞家族A成員1(MS4A1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1)48T/96TY16350Homo sapiens (Human)
銅離子轉(zhuǎn)運(yùn)ATP酶β肽(ATP7β)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for ATPase, Cu++ Transporting Beta Polypeptide (ATP7b)48T/96TY16351Homo sapiens (Human)
葡萄糖轉(zhuǎn)運(yùn)蛋白3(GLUT3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucose Transporter 3 (GLUT3)48T/96TY16352Rattus norvegicus (Rat)
腦指蛋白(BFP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Brain Finger Protein (BFP)48T/96TY16353Mus musculus (Mouse)
腫瘤壞死因子受體超家族成員4(TNFRSF4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4)48T/96TY16354Mus musculus (Mouse)
磷脂酰肌醇蛋白聚糖3(GPC3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glypican 3 (GPC3)48T/96TY16355Rattus norvegicus (Rat)
成纖維細(xì)胞生長因子8(FGF8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fibroblast Growth Factor 8, Androgen Induced (FGF8)48T/96TY16356Mus musculus (Mouse)
白介素18結(jié)合蛋白(IL18BP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Interleukin 18 Binding Protein (IL18BP)48T/96TY16357Rattus norvegicus (Rat)
紅細(xì)胞衍生核因子2樣蛋白2(NFE2L2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)48T/96TY16358Homo sapiens (Human)
糖皮質(zhì)激素誘導(dǎo)腫瘤壞死因子受體(GITR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Glucocorticoid Induced Tumor Necrosis Factor Receptor (GITR)48T/96TY16359Homo sapiens (Human)
端粒重復(fù)結(jié)合因子2相互作用蛋白(TERF2IP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for omeric Repeat Binding Factor 2 Interacting Protein (TERF2IP)48T/96TY16360Homo sapiens (Human)
類固醇5α還原酶2(SRD5α2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Steroid 5 Alpha Reductase 2 (SRD5a2)48T/96TY16361Rattus norvegicus (Rat)
頂體后致密板WW域結(jié)合蛋白(PAWP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Postacrosomal Sheath WW Domain Binding Protein (PAWP)48T/96TY16362Bos taurus; Bovine (Cattle)
天冬酰胺內(nèi)肽酶(LGMN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Legumain (LGMN)48T/96TY16363Mus musculus (Mouse)
三肽基肽酶Ⅰ(TPP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Tripeptidyl Peptidase I (TPP1)48T/96TY16364Mus musculus (Mouse)
組織蛋白酶A(CTSA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cathepsin A (CTSA)48T/96TY16365Mus musculus (Mouse)
前蛋白轉(zhuǎn)化酶枯草溶菌素1(PCSK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1)48T/96TY16366Rattus norvegicus (Rat)
鈉碘運(yùn)輸?shù)鞍祝∟IS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Sodium Iodide Symporter (NIS)48T/96TY16367Homo sapiens (Human)
Smad同源物3(Smad3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Mothers Against Decapentaplegic Homolog 3 (Smad3)48T/96TY16368Mus musculus (Mouse)
轉(zhuǎn)化生長因子β受體Ⅱ(TGFβR2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Transforming Growth Factor Beta Receptor II (TGFbR2)48T/96TY16369Mus musculus (Mouse)
集落刺激因子2受體β(CSF2Rβ)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Colony Stimulating Factor 2 Receptor Beta (CSF2Rb)48T/96TY16370Homo sapiens (Human)
δ樣蛋白1同源物(δLK1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Delta Like 1 Homolog (dLK1)48T/96TY16371Mus musculus (Mouse)
鳥苷酸環(huán)化酶激活因子2A(GUCA2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Guanylate Cyclase Activator 2A (GUCA2A)48T/96TY16372Mus musculus (Mouse)
骨膜蛋白(POSTN)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Periostin (POSTN)48T/96TY16373Rattus norvegicus (Rat)
NADH脫氫酶1(ND1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for NADH Dehydrogenase 1 (ND1)48T/96TY16374Rattus norvegicus (Rat)
胞漿多聚A結(jié)合蛋白1樣蛋白(PABPC1L)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Poly A Binding Protein Cytoplasmic 1 Like Protein (PABPC1L)48T/96TY16375Homo sapiens (Human)
含纈酪肽蛋白(VCP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Valosin Containing Protein (VCP)48T/96TY16376Rattus norvegicus (Rat)
周期素依賴性激酶8(CDK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 8 (CDK8)48T/96TY16377Homo sapiens (Human)
周期素依賴性激酶8(CDK8)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Cyclin Dependent Kinase 8 (CDK8)48T/96TY16378Mus musculus (Mouse)
低密度脂蛋白受體(LDLR)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Low Density Lipoprotein Receptor (LDLR)48T/96TY16379Mus musculus (Mouse)
脂肪細(xì)胞型脂肪酸結(jié)合蛋白(FABP4)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Fatty Acid Binding Protein 4, Adipocyte (FABP4)48T/96TY16380Mus musculus (Mouse)
維甲酸X受體α(RXRα)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Retinoid X Receptor Alpha (RXRa)48T/96TY16381Mus musculus (Mouse)
叉頭框蛋白P3(FOXP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Forkhead Box Protein P3 (FOXP3)48T/96TY16382Rattus norvegicus (Rat)
酪蛋白κ(CSN3)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Casein Kappa (CSN3)48T/96TY16383Bos taurus; Bovine (Cattle)
酪蛋白α(CSN1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)ELISA Kit for Casein Alpha (CSN1)48T/96TY16384Bos taurus; Bovine (Cattle)
各種類型ELISA測定時,一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。