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小鼠幽門螺旋菌IgG酶免試劑盒,(Hp-IgG)ELISA檢測試劑盒 ,英文名:Hp-IgG ELISA Kit ,英文縮寫:Hp-IgG ,常見試劑盒規(guī)格:96T/48T,產(chǎn)品別名:小鼠幽門螺旋菌IgGELISA檢測試劑盒,Hp-IgG 檢測試劑盒, Hp-IgG 檢測試劑盒
ELISA實(shí)驗(yàn)操作較繁雜,操作不當(dāng)將引起較大的誤差。小鼠幽門螺旋菌IgG酶免試劑盒,(Hp-IgG)ELISA檢測試劑盒在操作中應(yīng)注意以下5個(gè)方面。
1.隨著病情的進(jìn)展或由其他因素影響,某些抗體在機(jī)體內(nèi)的含量發(fā)生大幅波動(dòng),因此有必要對標(biāo)本進(jìn)行預(yù)處理,例如對血清標(biāo)本作適當(dāng)稀釋,或離心分離血脂等。間接法測定中,標(biāo)本適當(dāng)稀釋還可降低非特異性反應(yīng)。
2.加樣一般使用加液器,在ELISA中一般有4次加樣:加標(biāo)本、加酶結(jié)合物、加底物和加終止液。加標(biāo)本時(shí)必須每份標(biāo)本使用一支移液器吸嘴,以免交叉污染。加酶結(jié)合物、底物和終止液時(shí)則不需更換吸嘴。
3.在成批手工檢測中,還應(yīng)注意操作時(shí)差的影響。加樣及混勻時(shí)間的差異,使抗原抗體反應(yīng)和酶促反應(yīng)時(shí)間不*,對競爭法及定量檢測影響很大,不注意可能會導(dǎo)致錯(cuò)誤結(jié)果或定量不準(zhǔn)。
4.洗滌是ELISA操作過程中決定實(shí)驗(yàn)成敗的一個(gè)關(guān)鍵步驟。目的是除去未結(jié)合的免疫反應(yīng)物,終止抗原抗體反應(yīng),除去標(biāo)本中與反應(yīng)無關(guān)的成分、游離的酶標(biāo)物以及反應(yīng)過程中吸附于固相載體上的非特異性物質(zhì)。可用洗板機(jī)洗板或手工洗板,前者洗滌質(zhì)量較好,各反應(yīng)孔洗滌效果均一,批內(nèi)、批間差異小,手工洗板應(yīng)注意控制各孔洗滌效果,差異不宜太大。
5.準(zhǔn)確判定結(jié)果須使用ELISA測讀儀(酶標(biāo)儀),比色法判讀可選擇單波長或雙波長,根據(jù)反應(yīng)液顏色的不同選用不同波長的濾光片,以空白孔校零測定反應(yīng)孔的吸光度值(A值)。酶標(biāo)儀具有良好的重復(fù)性。
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白細(xì)胞介素24(IL24)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 24 (IL24)96TY17811Homo sapiens (Human)
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白細(xì)胞介素24(IL24)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 24 (IL24)96TY17813Rattus norvegicus (Rat)
白介素20(IL20)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 20 (IL20)96TY17814Homo sapiens (Human)
白介素29(IL29)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Interleukin 29 (IL29)96TY17815Homo sapiens (Human)
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S100鈣結(jié)合蛋白(S100)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for S100 Calcium Binding Protein (S100)96TY17822Homo sapiens (Human)
S100鈣結(jié)合蛋白B(S100B)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for S100 Calcium Binding Protein B (S100B)96TY17823Homo sapiens (Human)
硫氧化還原蛋白(Trx)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Thioredoxin (Trx)96TY17824Homo sapiens (Human)
凝血酶原片段F1+2(F1+2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Prothrombin Fragment 1+2 (F1+2)96TY17825Homo sapiens (Human)
凝血因子Ⅹ(F10)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Coagulation Factor X (F10)96TY17826Homo sapiens (Human)
蛋白酶激活受體2(PAR2)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Protease Activated Receptor 2 (PAR2)96TY17827Homo sapiens (Human)
血清淀粉樣蛋白A(SAA)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Serum Amyloid A (SAA)96TY17828Homo sapiens (Human)
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腦型肌酸激酶(CKB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Creatine Kinase, Brain (CKB)96TY17830Homo sapiens (Human)
磷脂酶D(PLD)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Phospholipase D (PLD)96TY17831Homo sapiens (Human)
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組織金屬蛋白酶抑制因子4(TIMP4)檢測試劑盒(化學(xué)發(fā)光免疫分析法)CLIA Kit for Tissue Inhibitors Of Metalloproteinase 4 (TIMP4)96TY17836Rattus norvegicus (Rat)
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各種類型ELISA測定時(shí),一般需經(jīng)過以下步驟:固相包被→加待測樣品→溫育→洗滌+加酶標(biāo)物→溫育→洗滌→加底物→溫育一加終止液→比色→判定結(jié)果。